Operational implications of manufacturing outsourcing for subcontractor plants: An empirical investigation

Purpose – The objectives of this paper are: to identify the key defining elements of a subcontractor plant from an operations management perspective and examine whether there are differences between the operational contexts of such plants and original equipment manufacturer (OEM) plants; and to examine whether these differences should translate into different operational practices, addressing the specific case of process quality management practices. Design/methodology/approach – The paper uses a multiple case study involving five plants in the electronics industry representative of the OEM and different subcontractor contexts. Findings – Results suggest that the operational contexts of subcontractor and OEM plants are different and that, as a result, these types of plants should emphasize different sets of process quality management practices. Research limitations/implications – Results are considered to be generalizable to most discrete goods industries. However, future research should ascertain whether these results replicate in industries other than electronics. Practical implications – OEMs, who have a critical role in disseminating best practice within the supply chain, must recognize the differences between OEM and subcontractor environments and avoid pushing one-size-fits-all best practice programs along the chain. Originality/value – Research in outsourcing to date has focused on the outsourcing decision per se and has mainly taken the perspective of the outsourcer firm. This study contributes to a better understanding of the operational implications of outsourcing decisions for subcontractor plants. It also responds to calls for more research linking quality management and supply chain management

Characterization of Hsp70 binding and nucleotide exchange by the yeast Hsp110 chaperone Sse1.

SSE1 and SSE2 encode the essential yeast members of the Hsp70-related Hsp110 molecular chaperone family. Both mammalian Hsp110 and the Sse proteins functionally interact with cognate cytosolic Hsp70s as nucleotide exchange factors. We demonstrate here that Sse1 forms high-affinity (Kd approximately 10-8 M) heterodimeric complexes with both yeast Ssa and mammalian Hsp70 chaperones and that binding of ATP to Sse1 is required for binding to Hsp70s. Sse1.Hsp70 heterodimerization confers resistance to exogenously added protease, indicative of conformational changes in Sse1 resulting in a more compact structure. The nucleotide binding domains of both Sse1/2 and the Hsp70s dictate interaction specificity and are sufficient for mediating heterodimerization with no discernible contribution from the peptide binding domains. In support of a strongly conserved functional interaction between Hsp110 and Hsp70, Sse1 is shown to associate with and promote nucleotide exchange on human Hsp70. Nucleotide exchange activity by Sse1 is physiologically significant, as deletion of both SSE1 and the Ssa ATPase stimulatory protein YDJ1 is synthetically lethal. The Hsp110 family must therefore be considered an essential component of Hsp70 chaperone biology in the eukaryotic cell

αvβ3 and α5β1 integrin-specific ligands: From tumor angiogenesis inhibitors to vascularization promoters in regenerative medicine?

Integrins are cell adhesion receptors predominantly important during normal and tumor angiogenesis. A sequence present on several extracellular matrix proteins composed of Arg-Gly-Asp (RGD) has attracted attention due to its role in cell adhesion mediated by integrins. The development of ligands that can bind to integrins involved in tumor angiogenesis and brake disease progression has resulted in new investigational drug entities reaching the clinical trial phase in humans. The use of integrin-specific ligands can be useful for the vascularization of regenerative medicine constructs, which remains a major limitation for translation into clinical practice. In order to enhance vascularization, immobilization of integrin-specific RGD peptidomimetics within constructs is a recommended approach, due to their high specificity and selectivity towards certain desired integrins. This review endeavours to address the potential of peptidomimetic-coated biomaterials as vascular network promoters for regenerative medicine purposes. Clinical studies involving molecules tracking active integrins in cancer angiogenesis and reasons for their failure are also addressed.Prémios Santa Casa Neurociências - Prize Melo e Castro for Spinal Cord Injury Research; Portuguese Foundation for Science and Technology [Doctoral fellowship (PD/BDE/127835/2016) to L. A. Rocha; IF Development Grant to A. J. Salgado; national funds through grant TUBITAK/0007/2014]. This article has been developed under the scope of the projects NORTE-01-0145-FEDER-000013, supported by the Northern Portugal Regional Operational Programme (NORTE 2020), under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (FEDER); This work has been funded by FEDER funds, through the Competitiveness Factors Operational Programme (COMPETE), and by National funds, through the Foundation for Science and Technology (FCT), under the scope of the project POCI-01-0145-FEDER-007038info:eu-repo/semantics/publishedVersio

A Fibre-Based Frame Element with Explicit Consideration of Bond-Slip Effects

Reinforced concrete (RC) frames subjected to seismic loading often depict localized member-end deformations due to strain penetration effects between adjacent members, such as beam-column and column-footing joints. Past experimental programs indicate that the bond-slip deformations occurring at the interface between the reinforcement and the surrounding concrete can contribute up to 40% of the lateral deformation of the RC members. The employment of advanced bond-slip models within detailed finite element formulations, capable of simulating continuous domains with highly discretized meshes, has witnessed great advances over the recent years with encouraging results. Nonetheless, this modelling approach is computationally heavy and hence inapplicable for practical seismic (nonlinear) analysis of structures. Alternatively, the use of beam-column elements with lumped or distributed plasticity is a more computationally efficient and engineering-friendly modelling approach. Unfortunately, the elements of this type available in conventional numerical packages did not yet consider an explicit simulation of the interface between the reinforcing bars and the surrounding concrete along their embedment length. The present study aimed at overcoming the foregoing limitation by developing an explicit bond-slip model applicable to general fibre-based beam-column elements. Using a state-of-the-art bond-slip constitutive model, the current paper introduces a zero-length element that computes the localized member-end deformations accounting for the bond-slip response at each reinforcing bar of a given RC section. Along with the material properties and anchorage conditions, the proposed nonlinear model also accounts for cyclic degradation and rebar yielding effects. Validation studies conducted with the proposed numerical formulation reveal a good agreement with past experimental tests, evidencing an important stability and accuracy at the expense of an acceptable additional computational effort.info:eu-repo/semantics/publishedVersio

Blind Prediction Tests as a Benchmark to Improve the Seismic Response of Fibre Models

The seismic behaviour of reinforced concrete framed structures involves a number of nonlinear material and geometrical phenomena that are impossible to model exhaustively in a single model. Furthermore, past studies showed that the most correct modelling options from the scientific viewpoint are sometimes challenged by experimental results. Over the years, attempts have been made to identify and measure the importance of different modelling options. This work intends to consolidate some of these findings and further extend them in order to progressively bridge the gap between solidly established theoretical principles and shaking table test results. The response of three different structures used in international blind prediction test challenges serves as benchmark to assess the goodness-of-fit of alternative numerical solutions. The interpretation of the results highlights the sensitivity of the response with respect to the modelling choices and provides indications towards the development of optimized numerical analyses.N/

Benefits of spine stabilization with biodegradable scaffolds in spinal cord injured rats

Spine stabilization upon spinal cord injury (SCI) is a standard procedure in clinical practice, but rarely employed in experimental models. Moreover, the application of biodegradable biomaterials for this would come as an advantage as it would eliminate the presence of a nondegradable prosthesis within the vertebral bone. Therefore, in the present work, we propose the use of a new biodegradable device specifically developed for spine stabilization in a rat model of SCI. A 3D scaffold based on a blend of starch with polycaprolactone was implanted, replacing delaminated vertebra, in male Wistar rats with a T8-T9 spinal hemisection. The impact of spinal stabilization on the locomotor behavior was then evaluated for a period of 12 weeks. Locomotor evaluation—assessed by Basso, Beatie, and Bresnahan test; rotarod; and open field analysis—revealed that injured rats subjected to spine stabilization significantly improved their motor performance, including higher coordination and rearing activity when compared with SCI rats without stabilization. Histological analysis further revealed that the presence of the scaffolds not only stabilized the area, but also simultaneously prevented the infiltration of the injury site by connective tissue. Overall, these results reveal that SCI stabilization using a biodegradable scaffold at the vertebral bone level leads to an improvement of the motor deficits and is a relevant element for the successful treatment of SCI.The authors would like to acknowledge the Portuguese Foundation for Science and Technology (Doctoral fellowship to Nuno Silva, SFRH/BD/40684/2007; Ciência 2007 Program to António Salgado; Grant N PTDC/SAU-BMA/114059/2009) and the Foundation Calouste de Gulbenkian to funds attributed to A.J. Salgado under the scope of the The Gulbenkian Programme to Support Cutting Edge Research in the Life Sciences

The Effects of Service Failure and Service Recovery on Customer Loyalty in E-Services: An Empirical Investigation

Although there has been a lot of research examining the effect of service failures and recovery on customer loyalty in traditional (bricks-and-mortar) services, there is still little rigorous empirical research examining this in e-service settings. The objective of this study is to empirically examine the impact of service failures and service recovery on customer loyalty in e-services. The study is based on data from an online questionnaire of customers of an e-banking service. The results validated the relationships generally found in traditional services: i) service failures result in decreased customer loyalty intentions; ii) effective recovery from failures increases customer loyalty intentions; and iii) unless recovery efforts elicit very high levels of satisfaction from customers, they can, at best, restore loyalty to the levels existing prior to the failures. The implications for the management of e-services are discussed

Charismatic, transformational and visionary dimensions in sport leadership : toward news paths for the study of coach-athletes relationships

In this paper we suggest possible applications of the charismatic, transformational and visionary leadership approaches to the sports field, to help understand the dynamics of the coach-athlete relationship. We present the results of our research using these concepts to analyze the work of sports managers. We note that few studies have focused on coaches as promoters of charismatic and transformational behaviors on athletes and teams